F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

SKU: FLU60

$218.00 USD; $218.00 USD
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Unit: 60μg 60μg 300μg Scrambled Control (60μg)

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Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.^[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.^[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.^[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.^[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.^[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.^[7]

F-CHP is labeled with fluorescein for direct fluorescence detection.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).^[3,7]

Applications: immunofluorescence,^[3] cell imaging,^[4] SDS-PAGE (in-gel western)^[7]

Features

More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
A non-antibody approach with no species restrictions against any co-staining antibody
Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
Stable in solution under 4 °C, eliminating the need to aliquot for storage

For research use only. Not intended or approved for diagnostic or therapeutic use.

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F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

$218.00

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F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

$218.00

Unit: 60μg 60μg 300μg Scrambled Control (60μg)

Product variants

Synonyms	F-CHP, collagen mimetic peptide (CMP)
Molecular weight	2952.01 g/mol
Purity	95% by HPLC
Conjugate	Single fluorescein tag per peptide
Excitation	494 nm
Emission	512 nm
Content	Purified lyophilized powder
Storage	-20 °C as powder, 4 °C after reconstitution in water

F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

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F-CHP | Collagen Hybridizing Peptide, 5-FAM Conjugate

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