R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.^[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.^[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.^[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.^[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.^[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.^[7]

R-CHP is labeled with sulfo-Cyanine3 for direct fluorescence detection.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).^[3,7]

Applications: immunofluorescence,^[3] cell imaging,^[4] SDS-PAGE (in-gel western)^[7]

Specification

Features

• More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
• High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
• Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
• Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
• A non-antibody approach with no species restrictions against any co-staining antibody
• Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
• Stable in solution under 4 °C, eliminating the need to aliquot for storage 

For research use only. Not intended or approved for diagnostic or therapeutic use.