Uncovering Fibrosis: Superior Sensitivity of CHPs in Targeting IPF
25 Jun 2024
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Paper: Adipose Stromal Cell-Secretome Counteracts Profibrotic Signals From IPF Lung Matrices
Idiopathic pulmonary fibrosis (IPF) is characterized by increased fibrosis in the lung over time, resulting in the lungs becoming stiffer and unable to function properly. In many cases IPF is not diagnosed until later stages, or is misdiagnosed for another disease whose symptoms largely overlap with IPF.
While it is well known that collagen levels increase in IPF pathology, it remains difficult to quantify using standard histological methods. Here, collagen hybridizing peptides (CHPs) were compared to standard collagen stains Masson’s trichrome in human IPF tissue samples and healthy controls. Using R-CHP, The Burgess group showed that CHPs outperformed Masson’s trichrome in detecting collagen deposition in tissues with IPF and interstitial lung disease compared to healthy controls.
This effect was consistent in native and decellularized tissues, where increased denatured collagen was noted in fibrotic tissues. This work highlights the utility of CHPs for detecting denatured collagen in IPF and other fibrotic conditions for its unparalleled sensitivity and ease of quantification.
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